T-2毒素对BALB/c小鼠营养物质表观消化率及小肠形态结构的影响

doi:10.11843/j.issn.0366-6964.2015.09.013

Abstract

Abstract:

This study was conducted to investigate the effect of T-2 toxin exposed to BALB/c mice on pH and moisture content in feces，apparent digestibility of nutrients，mineral elements and amino acids，as well as morphological structure in small intestine.A total of 80 BALB/c mice weighted about(20±2) g were randomly allotted to 4 groups(20 mice per group).The mice were fed one of four kinds of diets(0，0.4，1.0 and 2.5 mg•kg^-1•BW T-2 toxin exposed daily via intragastric administration for 28 d).The results showed as follows：the pH in feces in 1.0 mg•kg^-1•BW group was higher than that in control and 0.4 mg•kg^-1•BW groups(P<0.05)，pH in feces in 2.5 mg•kg^-1•BW group was the highest among all 4 groups(P<0.05 or P<0.01).The apparent digestibility of crude protein，ash，crude fiber and ether extract were greatly decreased with the increasing of T-2 toxin addition，and there were significant difference among different treatment groups(P<0.05 or P<0.01).The apparent digestibility of mineral elements(including Ca，Fe，Mg，Na and P) of 0.4 mg•kg^-1•BW group was largely reduced compared with control group(P<0.01).The mineral elements(including Ca，Zn，Mg，K，Mn and P) in 1.0 and 2.5 mg•kg^-1•BW groups were lower than those of control and 0.4 mg•kg^-1•BW groups(P>0.05 and P<0.01)，and 2.5 mg•kg^-1•BW group was significantly lower than 1.0 mg•kg^-1•BW group(P<0.01).The apparent digestibility of amino acid(including Asp，Thr，Ser，Glu，Gly，Ala，Val，Ile，Leu，Phe，Lys，His，Arg and Pro) in 1.0 mg•kg^-1•BW group were also observably declined compared with control or 0.4 mg•kg^-1•BW groups(P<0.05 and P<0.01)，and the digestibility of all kinds of amino acid in 2.5 mg•kg^-1•BW group were the lowest among all the 4 groups(P<0.01).Moreover，the duodenum，jejunum and ileum presented mucosal damage and microvilli exfoliated when the dose of T-2 toxin was more than 1.0 mg•kg^-1•BW.In addition，reduction of villous number and villous length，increase of crypt depth，decrease of the ratio of villus length to crypt depth were also observed in 1.0 and 2.5 mg•kg^-1•BW groups，and there were significant differences between 1.0,2.5 mg•kg^-1•BW groups,and control,0.4 mg•kg^-1•BW groups(P<0.05 and P<0.01)，respectively.It was concluded that the supplementation of T-2 toxin could damage intestinal mucosal，induce the increase of pH，decrease the apparent digestibility of nutrient，mineral elements and amino acid，and all these damage and decreasing were associated with dose-effect relationship of T-2 toxin.

CLC Number:

S815

YANG Jun-hua，CHEN Hui-ying，HAN Wei，ZHAO Zhi-hui，SUN Zhen-zhen，GUO Wen-bo. Influence of T-2 Toxin on Nutrient Apparent Digestibility and Small Intestinal Morphology in BALB/c Mice[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(9): 1584-1592.

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Influence of T-2 Toxin on Nutrient Apparent Digestibility and Small Intestinal Morphology in BALB/c Mice

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